ID: 1017 Extracellular vesicles of ETV2 transfected fibroblasts stimulate endothelial cells and improve neovascularization in a murine model of hindlimb ischemia

Authors

  • Phuc Van Pham Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University HCMC, Ho Chi Minh City, Vietnam
  • Ngoc Bich Vu Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University HCMC, Ho Chi Minh City, Vietnam
  • Thuy Thi-Thanh Dao Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University HCMC, Ho Chi Minh City, Vietnam
  • Ha Thi-Ngan Le Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University HCMC, Ho Chi Minh City, Vietnam
  • Lan Thi Phi Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University HCMC, Ho Chi Minh City, Vietnam
  • Oanh Thuy Huynh Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University HCMC, Ho Chi Minh City, Vietnam
  • Mai Thi-Hoang Truong Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University HCMC, Ho Chi Minh City, Vietnam
  • Oanh Thi-Kieu Nguyen Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University HCMC, Ho Chi Minh City, Vietnam
  • Ngoc Kim Phan Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University, Ho Chi Minh city Viet Nam

DOI:

https://doi.org/10.15419/bmrat.v4iS.295

Keywords:

Extracellular vesicles, EVs, ETV2, Fibroblasts, Ischemia, Hindlimb ischemia, biology

Abstract

Ischemia are common conditions related to lack of blood supply to tissues. Depending on the ischemic sites, ischemia can cause different diseases, such as hindlimb ischemia, heart infarction and stroke. This study aims to evaluate how extracellular vesicles (EVs) derived from ETV2 transfected fibroblasts affect endothelial cell proliferation and neovascularization in a murine model of hindlimb ischemia. Human fibroblasts were isolated and cultured under standard conditions and expanded to the 3th passage before use in experiments. Human fibroblasts were transduced with a viral vector containing the ETV2 gene. Transduced cells were selected by puromycin treatment. These cells were further cultured for collection of EVs, which were isolated from culture supernatant. Following co-culture with endothelial cells, EVs were evaluated for their effect on endothelial cell proliferation and were directly injected into ischemic tissues of a murine model of hindlimb ischemia. The results showed that EVs could induce endothelial cell proliferation in vitro and improved neovascularization in a murine model of hindlimb ischemia. Our results suggest that EVs derived from ETV2-transfected fibroblasts can be promising non-cellular products for the regeneration of blood vessels.

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Published

2017-09-05

How to Cite

ID: 1017 Extracellular vesicles of ETV2 transfected fibroblasts stimulate endothelial cells and improve neovascularization in a murine model of hindlimb ischemia. (2017). Biomedical Research and Therapy, 4(S), S 92. https://doi.org/10.15419/bmrat.v4iS.295

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