Cytokine profile indicators in rat blood serum in a model of esophagus burn induced by antioxidant chemical preparation
DOI:
https://doi.org/10.15419/bmrat.v4i9.367Keywords:
Anti-inflammatory, Burn the esophagus, Inflammatory, Interleukins, MelaninAbstract
Background: According to modern concepts of burn disease development, one of the main burn complications is inflammation occurrence. Despite the large number of drugs used to treat the effects of chemical burns of the esophagus, we find conflicting information in the literature about their effectiveness. Moreover the problem of burn wound healing process quickening, as well as the prevention measures against possible post burn complications are really relevant if we aim to subjective portability of rehabilitation period facilitation.
Methods: Study the immune system cytokine levels in immature rat blood serum under the simulation of 1 and 2 degree esophageal chemical burns in case of melanin administration as a drug. Was estimated ELISA using sets of reagents and instructions from Biotrack ELISA System company «Healthcare».
Results: The significant increase of pro-inflammatory cytokines (PC) was shown mostly on day 7 of the experiment, which reflects the activity and severity of the disease process. In terms of further research the level of pro-inflammatory PC can be multidirectional. The significant decrease of anti-inflammatory cytokines amount was found under conditions of AEB at 15th and 21st days. At 1st and 2nd degree ABE the observed changes were multidirectional. Melanin administration resulted in the fast decrease of blood levels of all cytokines in our experiment to the values close to normal in conditions of esophagus chemical burn progress.
Conclusion: Therefore, it was shown that some factors have the ability of changing the levels of pro inflammatory and anti-inflammatory cytokines in the models 1st and 2nd degree ABE and AEB in immature rats. The obtained data showed, that 1st degree ABE progressed without septic complications. In the models of 2nd degree ABE and AEB, the risk of poly organic failure remains. Melanin administration resulted in the fast decrease of blood levels of all cytokines in our experiment to the values close to normal in conditions of esophagus chemical burn progress.
References
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<li class="show">Beregova T.V., S.A.A., Neporada K.S (2014). The impact on the activity of melanin no-ergic system in the tissues of the salivary glands under the conditions hiperhastrynemiyi. World medicine and biology 1.</li>
<li class="show">Brenner, M., & Hearing, V. J. (2008). The protective role of melanin against UV damage in human skin. Photochemistry and Photobiology, 84(3), 539–549. <a href="https://doi.org/10.1111/j.1751-1097.2007.00226.x ">https://doi.org/10.1111/j.1751-1097.2007.00226.x </a>PMID:18435612</li>
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<li class="show">Chornenka, N. M. (2016). R.Y.B., Savchuk OM, Torgalo EO, Beregova TV, and Ostapchenko LI. Correction Parameters of Endogenous Intoxication in Experimental Burn Disease at the Stage of Toxemia. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7, 1042–1047.</li>
<li class="show">Chyzhanska, N., Tsyryuk, O., and Beregova, T. (2007). The level of cortisol in the blood of rats before and after stress action against the background of melanin. Visnik of problems of biology and medicine 1, 40-44.</li>
<li class="show">Falalyeyeva T.M., T.O.I., Chyzhanskaya N.V., Zharova V.P. (2009). The influence of melanin on cortisol blood level in the rats in conditions of stress action. Ukrainian Antarctic Journal, 391-394.</li>
<li class="show">Fistal, E. Y. G. P. K. (2004). G.E Samoilenko et al. Kharkov: Combustiology.</li>
<li class="show">Gelashvili (2008). Option of periodization of biologically similar stages of human and rats ontogenesis. Saratov scientific-medical journal 4.</li>
<li class="show">Golyshkin, D., Falalyeyeva, T., Chyzhanska, N., and Beregova, T. (2015). LI Ostapchenko White blood count of rats under stress-induced stomach lesions and the prophylactic administration of melanin. Ukrainian Antarctic Journal, 114.</li>
<li class="show">Gorbach O., K.N., Skachkova O. (2013). Immunological biomarkers as surrogates for clinical response for dendritic cells vaccine therapy for non-small cell lung cancer and renal cell carcinoma. Paper presented at: Materials of Joint European Cancer Congress (Amsterdam, Netherlands).</li>
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<li class="show">Holyshkin, D. V. (2014). F.T.N., Neporada K.S, Beregova T.V. Influence of melanin on the state of the gastric mucosa and the reaction of the hypothalamic-pituitary-adrenal axis under conditions of acute stress action. The Journal of Physiology, 61, 65–72.</li>
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<li class="show">Keypour, S., Riahi, H., Moradali, M.-F., & Rafati, H. (2008). Investigation of the antibacterial activity of a chloroform extract of Ling Zhi or Reishi medicinal mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst.(Aphyllophoromycetideae), from Iran. International Journal of Medicinal Mushrooms, 10.</li>
<li class="show">Klimenko M.O, N.L.G. (2009). Burn disease (pathogenesis and treatment).</li>
<li class="show">Köller, M., David, A., Hahn, M., and Muhr, G. (1997). Liberation of interleukin 12 (IL12) after trauma and polytrauma. Paper presented at: Langenbecks Archiv fur Chirurgie Supplement Kongressband Deutsche Gesellschaft fur Chirurgie Kongress.</li>
<li class="show">Kovalenko, O.M., Mal'tsev, D.V., Kazmirchuk, V., and Kovalenko, A.O. (2014). [Studying of cytokine dynamics in injured persons with severe burns for estimation of severity and prognosis]. Klinichna khirurhiia, 49-53.</li>
<li class="show">Lyons, A., Kelly, J. L., Rodrick, M. L., Mannick, J. A., & Lederer, J. A. (1997). Major injury induces increased production of interleukin-10 by cells of the immune system with a negative impact on resistance to infection. Annals of Surgery, 226(4), 450–458. <a href="https://doi.org/10.1097/00000658-199710000-00006">https://doi.org/10.1097/00000658-199710000-00006</a> PMID:9351713</li>
<li class="show">Matevosyan J. R., M.H.A. (2013). The influence of interleukin-1β and its receptor antagonist on healing of pyloroduodenal ulcers. Medicine science Armenia. 53 (3):130-133.</li>
<li class="show">Messingham, K. A., Heinrich, S. A., & Kovacs, E. J. (2001). Estrogen restores cellular immunity in injured male mice via suppression of interleukin-6 production. Journal of Leukocyte Biology, 70(6), 887–895. PMID:11739551</li>
<li class="show">Napolitano, L. M., & Campbell, C. (1994). Nitric oxide inhibition normalizes splenocyte interleukin-10 synthesis in murine thermal injury. Archives of Surgery (Chicago, Ill.), 129(12), 1276–1282. <a href="https://doi.org/10.1001/archsurg.1994.01420360066008">https://doi.org/10.1001/archsurg.1994.01420360066008</a> PMID:7986156</li>
<li class="show">Paunel-Görgülü, A., Kirichevska, T., Lögters, T., Windolf, J., & Flohé, S. (2012). Molecular mechanisms underlying delayed apoptosis in neutrophils from multiple trauma patients with and without sepsis. Molecular Medicine (Cambridge, Mass.), 18(3), 325–335. <a href="https://doi.org/10.2119/molmed.2011.00380">https://doi.org/10.2119/molmed.2011.00380</a> PMID:22231730</li>
<li class="show">Pileri, D., Accardo Palombo, A., D’Amelio, L., D’Arpa, N., Amato, G., Masellis, A., . . . Conte, C. (2008). Concentrations of cytokines IL-6 and IL-10 in plasma of burn patients: Their relationship to sepsis and outcome. Annals of Burns and Fire Disasters, 21(4), 182–185. PMID:21991134</li>
<li class="show">Polikarpov A.V., P.E.E. (2011). Comparative study of the dynamics of pro-inflammatory and anti-inflammatory cytokine levels in skin burns razlichnoyprirody. Bulletin of the University of Kharkiv Series: Biology., 971, 27–32.</li>
<li class="show">Racca, S., Spaccamiglio, A., Esculapio, P., Abbadessa, G., Cangemi, L., DiCarlo, F., & Portaleone, P. (2005). Effects of swim stress and α-MSH acute pre-treatment on brain 5-HT transporter and corticosterone receptor. Pharmacology, Biochemistry, and Behavior, 81(4), 894–900. <a href="https://doi.org/10.1016/j.pbb.2005.06.014">https://doi.org/10.1016/j.pbb.2005.06.014</a> PMID:16023186</li>
<li class="show">Raetska Ya.B., T.V.I., O.M. Savchuk, L.I. Ostapchenko. (2013). Experimental modeling of first-degree chemically-induced esophageal burns in rats. Medicinal Chemistry (Shariqah, United Arab Emirates), 15, 30–34.</li>
<li class="show">Raetska, Y. B. (2016). Proinflammatory and anti-inflammatory cytokines level in rat serum under modelinf of esophagus alkali burns of 1st and 2nd degrees. Scientific Journal Visnik Biology., 70, 67–70.</li>
<li class="show">Reva I.V., R.G.V., Yamamoto T., et. all. (2013). Interaction of immunocites in the reparative regeneration of the skin. Basic research. 453-459.</li>
<li class="show">Romanovskaia, V., Tashirev, A., Shilin, S., and Chernaia, N. (2010). Resistance to UV radiation of microorganisms isolated from the rock biotopes of the Antarctic region. Mikrobiolohichnyi zhurnal (Kiev, Ukraine: 1993) 72, 8.</li>
<li class="show">Seniuk O. F., G.L.F., Palamar L. A., Krul N. I. (2014). Effects of melanin-glucan complex, isolated from polypore fungi, on the lifespan of female icr mice. Probl aging and longevity 23, 11—27.</li>
<li class="show">Seniuk, O.F., Gorovoj, L.F., Kovalev, V., Palamar, L.A., Krul, N.I., and Kurchenko, V.P. (2009). Anticarcinogenic Properties of Melanin-Glucan Complex from Higher Fungi. Paper presented at: Proceedings of the 5~(th) International Medicinal Mushroom Conference.</li>
<li class="show">Ward, P. A., & Lentsch, A. B. (1999). The acute inflammatory response and its regulation. Archives of Surgery (Chicago, Ill.), 134(6), 666–669. <a href="https://doi.org/10.1001/archsurg.134.6.666">https://doi.org/10.1001/archsurg.134.6.666</a> PMID:10367878</li>
<li class="show">Wick, M., Kollig, E., Walz, M., Muhr, G., and Köller, M. (2000). Does liberation of interleukin-12 correlate with the clinical course of polytraumatized patients? Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen 71, 1126-1131.</li>
<li class="show">Zhou, H., Tu, J., Huang, Y., Chen, X., and Deng, Y. (2012). Changes in serum contents of interleukin-6 and interleukin-10 and their relation with occurrence of sepsis and prognosis of severely burned patients.</li>
<li class="show">Zhonghua shao shang za zhi= Zhonghua shaoshang zazhi= Chinese journal of burns 28, 111-115.</li>
<li class="show">Zmushko EI, B.E., Mitin Yu (2001). Clinical immunology. Guidelines for doctors. (CПб).</li>
</ol>
<li class="show">Agay, D., Andriollo-Sanchez, M., Claeyssen, R., Touvard, L., Denis, J., Roussel, A. M., & Chancerelle, Y. (2008). Interleukin-6, TNF-alpha and interleukin-1 beta levels in blood and tissue in severely burned rats. European Cytokine Network, 19(1), 1–7. PMID:18299267</li>
<li class="show">Belardelli, F. (1995). Role of interferons and other cytokines in the regulation of the immune response. APMIS, 103(3), 161–179. <a href="https://doi.org/10.1111/j.1699-0463.1995.tb01092.x">https://doi.org/10.1111/j.1699-0463.1995.tb01092.x</a> PMID:7538771</li>
<li class="show">Beregova T.V., S.A.A., Neporada K.S (2014). The impact on the activity of melanin no-ergic system in the tissues of the salivary glands under the conditions hiperhastrynemiyi. World medicine and biology 1.</li>
<li class="show">Brenner, M., & Hearing, V. J. (2008). The protective role of melanin against UV damage in human skin. Photochemistry and Photobiology, 84(3), 539–549. <a href="https://doi.org/10.1111/j.1751-1097.2007.00226.x ">https://doi.org/10.1111/j.1751-1097.2007.00226.x </a>PMID:18435612</li>
<li class="show">Chornenka N.M., Y.B.R., O.M. Savchuk, E.O. Torgalo, T.V. Beregova, L.I. Ostapchenko. (2016). Correction Parameters of Endogenous Intoxication in Experimental Burn Disease at the Stage of Toxemia. RJPBCS 7, 1042.</li>
<li class="show">Chornenka, N. M. (2016). R.Y.B., Savchuk OM, Torgalo EO, Beregova TV, and Ostapchenko LI. Correction Parameters of Endogenous Intoxication in Experimental Burn Disease at the Stage of Toxemia. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7, 1042–1047.</li>
<li class="show">Chyzhanska, N., Tsyryuk, O., and Beregova, T. (2007). The level of cortisol in the blood of rats before and after stress action against the background of melanin. Visnik of problems of biology and medicine 1, 40-44.</li>
<li class="show">Falalyeyeva T.M., T.O.I., Chyzhanskaya N.V., Zharova V.P. (2009). The influence of melanin on cortisol blood level in the rats in conditions of stress action. Ukrainian Antarctic Journal, 391-394.</li>
<li class="show">Fistal, E. Y. G. P. K. (2004). G.E Samoilenko et al. Kharkov: Combustiology.</li>
<li class="show">Gelashvili (2008). Option of periodization of biologically similar stages of human and rats ontogenesis. Saratov scientific-medical journal 4.</li>
<li class="show">Golyshkin, D., Falalyeyeva, T., Chyzhanska, N., and Beregova, T. (2015). LI Ostapchenko White blood count of rats under stress-induced stomach lesions and the prophylactic administration of melanin. Ukrainian Antarctic Journal, 114.</li>
<li class="show">Gorbach O., K.N., Skachkova O. (2013). Immunological biomarkers as surrogates for clinical response for dendritic cells vaccine therapy for non-small cell lung cancer and renal cell carcinoma. Paper presented at: Materials of Joint European Cancer Congress (Amsterdam, Netherlands).</li>
<li class="show">Hetyuhaylo (2007). Mechanisms of burn disease and justification of the drug "Kriohor" for its treatment. In Pathologic Physiology (Kharkiv: Kharkiv).</li>
<li class="show">Holyshkin, D. V. (2014). F.T.N., Neporada K.S, Beregova T.V. Influence of melanin on the state of the gastric mucosa and the reaction of the hypothalamic-pituitary-adrenal axis under conditions of acute stress action. The Journal of Physiology, 61, 65–72.</li>
<li class="show">Kazakov VN, M.B., Bullfinch AG et al. Ways of interaction between the nervous, endocrine and immune systems in the body's regulation of funktsiy. Archive for Clinical and Experimental Medicine 13, 3-10.</li>
<li class="show">Keypour, S., Riahi, H., Moradali, M.-F., & Rafati, H. (2008). Investigation of the antibacterial activity of a chloroform extract of Ling Zhi or Reishi medicinal mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst.(Aphyllophoromycetideae), from Iran. International Journal of Medicinal Mushrooms, 10.</li>
<li class="show">Klimenko M.O, N.L.G. (2009). Burn disease (pathogenesis and treatment).</li>
<li class="show">Köller, M., David, A., Hahn, M., and Muhr, G. (1997). Liberation of interleukin 12 (IL12) after trauma and polytrauma. Paper presented at: Langenbecks Archiv fur Chirurgie Supplement Kongressband Deutsche Gesellschaft fur Chirurgie Kongress.</li>
<li class="show">Kovalenko, O.M., Mal'tsev, D.V., Kazmirchuk, V., and Kovalenko, A.O. (2014). [Studying of cytokine dynamics in injured persons with severe burns for estimation of severity and prognosis]. Klinichna khirurhiia, 49-53.</li>
<li class="show">Lyons, A., Kelly, J. L., Rodrick, M. L., Mannick, J. A., & Lederer, J. A. (1997). Major injury induces increased production of interleukin-10 by cells of the immune system with a negative impact on resistance to infection. Annals of Surgery, 226(4), 450–458. <a href="https://doi.org/10.1097/00000658-199710000-00006">https://doi.org/10.1097/00000658-199710000-00006</a> PMID:9351713</li>
<li class="show">Matevosyan J. R., M.H.A. (2013). The influence of interleukin-1β and its receptor antagonist on healing of pyloroduodenal ulcers. Medicine science Armenia. 53 (3):130-133.</li>
<li class="show">Messingham, K. A., Heinrich, S. A., & Kovacs, E. J. (2001). Estrogen restores cellular immunity in injured male mice via suppression of interleukin-6 production. Journal of Leukocyte Biology, 70(6), 887–895. PMID:11739551</li>
<li class="show">Napolitano, L. M., & Campbell, C. (1994). Nitric oxide inhibition normalizes splenocyte interleukin-10 synthesis in murine thermal injury. Archives of Surgery (Chicago, Ill.), 129(12), 1276–1282. <a href="https://doi.org/10.1001/archsurg.1994.01420360066008">https://doi.org/10.1001/archsurg.1994.01420360066008</a> PMID:7986156</li>
<li class="show">Paunel-Görgülü, A., Kirichevska, T., Lögters, T., Windolf, J., & Flohé, S. (2012). Molecular mechanisms underlying delayed apoptosis in neutrophils from multiple trauma patients with and without sepsis. Molecular Medicine (Cambridge, Mass.), 18(3), 325–335. <a href="https://doi.org/10.2119/molmed.2011.00380">https://doi.org/10.2119/molmed.2011.00380</a> PMID:22231730</li>
<li class="show">Pileri, D., Accardo Palombo, A., D’Amelio, L., D’Arpa, N., Amato, G., Masellis, A., . . . Conte, C. (2008). Concentrations of cytokines IL-6 and IL-10 in plasma of burn patients: Their relationship to sepsis and outcome. Annals of Burns and Fire Disasters, 21(4), 182–185. PMID:21991134</li>
<li class="show">Polikarpov A.V., P.E.E. (2011). Comparative study of the dynamics of pro-inflammatory and anti-inflammatory cytokine levels in skin burns razlichnoyprirody. Bulletin of the University of Kharkiv Series: Biology., 971, 27–32.</li>
<li class="show">Racca, S., Spaccamiglio, A., Esculapio, P., Abbadessa, G., Cangemi, L., DiCarlo, F., & Portaleone, P. (2005). Effects of swim stress and α-MSH acute pre-treatment on brain 5-HT transporter and corticosterone receptor. Pharmacology, Biochemistry, and Behavior, 81(4), 894–900. <a href="https://doi.org/10.1016/j.pbb.2005.06.014">https://doi.org/10.1016/j.pbb.2005.06.014</a> PMID:16023186</li>
<li class="show">Raetska Ya.B., T.V.I., O.M. Savchuk, L.I. Ostapchenko. (2013). Experimental modeling of first-degree chemically-induced esophageal burns in rats. Medicinal Chemistry (Shariqah, United Arab Emirates), 15, 30–34.</li>
<li class="show">Raetska, Y. B. (2016). Proinflammatory and anti-inflammatory cytokines level in rat serum under modelinf of esophagus alkali burns of 1st and 2nd degrees. Scientific Journal Visnik Biology., 70, 67–70.</li>
<li class="show">Reva I.V., R.G.V., Yamamoto T., et. all. (2013). Interaction of immunocites in the reparative regeneration of the skin. Basic research. 453-459.</li>
<li class="show">Romanovskaia, V., Tashirev, A., Shilin, S., and Chernaia, N. (2010). Resistance to UV radiation of microorganisms isolated from the rock biotopes of the Antarctic region. Mikrobiolohichnyi zhurnal (Kiev, Ukraine: 1993) 72, 8.</li>
<li class="show">Seniuk O. F., G.L.F., Palamar L. A., Krul N. I. (2014). Effects of melanin-glucan complex, isolated from polypore fungi, on the lifespan of female icr mice. Probl aging and longevity 23, 11—27.</li>
<li class="show">Seniuk, O.F., Gorovoj, L.F., Kovalev, V., Palamar, L.A., Krul, N.I., and Kurchenko, V.P. (2009). Anticarcinogenic Properties of Melanin-Glucan Complex from Higher Fungi. Paper presented at: Proceedings of the 5~(th) International Medicinal Mushroom Conference.</li>
<li class="show">Ward, P. A., & Lentsch, A. B. (1999). The acute inflammatory response and its regulation. Archives of Surgery (Chicago, Ill.), 134(6), 666–669. <a href="https://doi.org/10.1001/archsurg.134.6.666">https://doi.org/10.1001/archsurg.134.6.666</a> PMID:10367878</li>
<li class="show">Wick, M., Kollig, E., Walz, M., Muhr, G., and Köller, M. (2000). Does liberation of interleukin-12 correlate with the clinical course of polytraumatized patients? Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen 71, 1126-1131.</li>
<li class="show">Zhou, H., Tu, J., Huang, Y., Chen, X., and Deng, Y. (2012). Changes in serum contents of interleukin-6 and interleukin-10 and their relation with occurrence of sepsis and prognosis of severely burned patients.</li>
<li class="show">Zhonghua shao shang za zhi= Zhonghua shaoshang zazhi= Chinese journal of burns 28, 111-115.</li>
<li class="show">Zmushko EI, B.E., Mitin Yu (2001). Clinical immunology. Guidelines for doctors. (CПб).</li>
</ol>
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2017-09-20
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How to Cite
Cytokine profile indicators in rat blood serum in a model of esophagus burn induced by antioxidant chemical preparation. (2017). Biomedical Research and Therapy, 4(9), 1591-1606. https://doi.org/10.15419/bmrat.v4i9.367
