Mesenchymal stem cells accelerate liver regeneration in acute liver failure animal model

Authors

  • Agung Putra Stem Cell And Cancer Research (SCCR), Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central Java, Indonesia; Department of Postgraduate Biomedical Science, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central Java, Indonesia; Department of Pathological Anatomy, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central Java, Indonesia http://orcid.org/0000-0002-9822-3119
  • Arini Dewi Antari Department of Pathological Anatomy, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central Java, Indonesia
  • Azizah Retno Kustiyah Department of Pediatric, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central Java, Indonesia
  • Yulice Soraya Nur Intan Department of Surgical, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central Java, Indonesia
  • Nur Anna C. Sadyah Department of Internal Medicine, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central Java, Indonesia
  • Nugraha Wirawan Bachelor of Medical Science Program, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central Java, Indonesia
  • Samara Astarina Bachelor of Medical Science Program, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central Java, Indonesia
  • Nasrul Zubir Department of Gastroenterohepatology, Medical Faculty, Andalas University, Padang, West Sumatera, Indonesia
  • Delfitri Munir Faculty of Medicine, Sumatera Utara University, Medan, North Sumatera, Indonesia; Pusat Unggulan Inovasi (PUI) Stem Cell, Sumatera Utara University (USU), Medan, North Sumatera, Indonesia

DOI:

https://doi.org/10.15419/bmrat.v5i11.498

Keywords:

ALF, MSCs, PDGF, SGOT, SGPT, VEGF

Abstract

Introduction: The massive hepatic necrosis of acute liver failure (ALF) results in a sudden loss of hepatic cells. Although most hepatocyte cells of ALF are completely lost, stem cell-derived circulating cells and endogenous progenitor cells rapidly regenerate them. Mesenchymal stem cells (MSCs) have a critical role in the regeneration of liver injury through regulating platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) levels. However, their fluctuating levels in the healing process and correlation to the decrease of liver function markers remain unclear. The aim of this study was to analyze the effects of MSCs in accelerating liver regeneration of ALF by measuring VEGF and PDGF levels on day 2 and 7, as well as SGPT and SGOT levels, and assessing histopathology appearance.

Methods: Using an ALF rat model, 12 animals were randomly assigned into two groups: umbilical cord (UC)-MSC injection (T1) and vehicle control (Veh). ELISA assay was employed to measure PDGF and VEGF levels, an automatic analyzer was used to assess serum glutamic pyruvic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT), and hematoxylin and eosin (H&E) staining was used to evaluate morphological appearance.

Results: The study showed an significant (P<0.001) increase of PDGF and VEGF levels on the 2nd day, followed by a decrease on the 7th day, along with a decrease of SGPT and SGOT levels as well as the normality of histology appearance.

Conclusion: In conclusion, administration of MSCs may accelerate liver regeneration of ALF through PDGF and VEGF regulation.

 

Published

2018-11-14

Issue

Section

Original Research

How to Cite

Mesenchymal stem cells accelerate liver regeneration in acute liver failure animal model. (2018). Biomedical Research and Therapy, 5(11), 2802-2810. https://doi.org/10.15419/bmrat.v5i11.498

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