Study on gene expression of IL-17A in eutopic and ectopic tissue sample of endometriosis patients and comparison with control group

Authors

  • Fakhri Hoseininasab Department of Immunology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Yahya Vahidi Mehrjardi Department of Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Atiyeh Javaheri Department of Obstetrics and Gynecology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Masoud Tajamolian Department of Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Morteza Samadi Department of Immunology, school of medicine, Isfahan university of medical sciences, Isfahan, Iran

DOI:

https://doi.org/10.15419/bmrat.v11i6.894

Keywords:

endometriosis, IL-17A, Th17 lymphocytes, inflammatory

Abstract

Introduction: The growth of endometrial tissue beyond the uterine cavity characterizes endometriosis, a chronic inflammatory disease. IL-17A, along with other immune mediators and a myriad of immune cells including Th17 lymphocytes and macrophages, are more prevalent in the peritoneal fluid of patients with endometriosis. IL–17A facilitates the implantation of endometrial tissue in ectopic locations by inducing growth factors and inflammatory cytokines from other immune cells. This work focuses on the levels of IL-17A gene expression in tissue samples from patient and control groups, as prior research has not extensively covered the gene expression of inflammatory mediators in tissue samples.

Methods: This study is both experimental and case-control in nature. Tissue samples were collected from 32 women undergoing laparoscopy at Yazd Shahid Sadoughi Hospital. The samples were divided into two groups: 16 patients with endometriosis and 16 healthy individuals. Within the patient group, there were two types of samples: eutopic and ectopic. The expression of IL-17A was assessed with RT-qPCR.

Results: RT-qPCR results showed a significant difference in IL-17A gene expression between ectopic lesions and eutopic samples from endometriosis patients (P value < 0.0001). Additionally, there was a significant increase in gene expression in eutopic samples from endometriosis patients compared to control samples (P value = 0.0176).

Conclusion: This study confirms the crucial involvement of inflammatory mediators like IL-17A in endometriosis through molecular findings in tissue samples, reflecting the elevated IL-17A content in the eutopic endometrium and ectopic lesions of endometriosis patients.

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Published

2024-06-30

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How to Cite

Study on gene expression of IL-17A in eutopic and ectopic tissue sample of endometriosis patients and comparison with control group. (2024). Biomedical Research and Therapy, 11(6), 6482-6487. https://doi.org/10.15419/bmrat.v11i6.894

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