Isolation of endothelial progenitor cells from human adipose tissue

Authors

  • Phuc Van Pham Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University, Ho Chi Minh city, Vietnam; Faculty of Biology-Biotechnology, University of Science, Vietnam National University, Ho Chi Minh city, Vietnam http://orcid.org/0000-0001-7254-0717
  • Ngoc Bich Vu Laboratory of Stem Cell Research and Application, University of Science, Viet Nam National University, Ho Chi Minh city, Viet Nam
  • Hoa Trong Nguyen Laboratory of Stem Cell Research and Application, University of Science, Viet Nam National University, Ho Chi Minh city, Viet Nam
  • Ngoc Kim Phan Laboratory of Stem Cell Research and Application, University of Science, Viet Nam National University, Ho Chi Minh city, Viet Nam

DOI:

https://doi.org/10.15419/bmrat.v3i05.98

Abstract

Adipose tissue is a rich source of stem cells, especially mesenchymal stem cells (MSCs). This study aimed to identify and isolate endothelial progenitor cells (EPCs) from human adipose tissue. Belly adipose tissues were collected from donors with consent. Stromal vascular fractions (SVFs) were extracted from adipose tissues by enzyme collagenase using commercial kits. SVFs were cultured in MSCCult medium for 24 h to obtain MSCs, then supernatant was collected and cell pellet cultured in EGM-2 medium to obtain adipose tissue EPCs (ADEPCs). ADEPCs were checked for surface marker expression of CD31 and VEGFR2, and for angiogenesis capability in vitro. The results showed that SVFs contained a pool of EPCs with strong angiogenesis potential and that adipose tissue is not only a source for MSCs but also for EPCs. Therefore, ADEPCs may a useful source of EPCs for vascular medicine.

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Published

2016-05-27

Issue

Vol. 3 No. 05 (2016)

Section

Original Research

License

Copyright The Author(s) 2017. This article is published with open access by BioMedPress. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

How to Cite

Isolation of endothelial progenitor cells from human adipose tissue. (2016). Biomedical Research and Therapy, 3(05), 645-652. https://doi.org/10.15419/bmrat.v3i05.98