Circulating apoptotic endothelial cell-derived microparticles are predicted metabolically unhealthy obesity
DOI:
https://doi.org/10.15419/bmrat.v4i1.147Keywords:
Endothelial cell-derived micro particles, Insulin resistance, Metabolically healthy obesity, Metabolically unhealthy obesityAbstract
Introduction: Circulating apoptotic endothelial cell-derived micro particles (EMPs) are a marker of endothelial dysfunction and cardiovascular (CV) risk in type 2 diabetes mellitus patients. There is evidence regarding association between apoptotic EMP number and CV disease in obese individuals. The aim of the study to investigate whether increased number of circulating apoptotic EMPs may predict transformation of Met-HO into Met-UHO.
Methods: The study was retrospectively evolved 89 patients with established abdominal obesity (47 patients with Met-UHO determined as MetS and 42 subjects with Met-HO) from the large cohort of abdominal obesity patients (n=268). Thirty five healthy volunteers matched for age and sex were involved in the study as a control cohort. Obesity-related biomarker (adiponectin, leptin, vistafin) and EMPs were measured at baseline. Flow cytometry was used to determine EMPs with immune phenotype CD31+/annexin V+ and CD144+/annexin V+.
Results: There was not found a significant difference between numbers of EMPs labeled CD31+/ Annexin V+ in Met-UHO and Met-HO patients, while Met-UHO patients had a significantly increased level of circulating CD144+/ Annexin V+ compared with Met-HO individuals. Multivariate logistic regression analysis has revealed the HOMA-IR, number of CV risk factors, serum leptin and hs-CRP independently predicted numbers of circulating CD31+/ Annexin V+ and CD144+/ Annexin V+ EMPs in Met-UHO. In Met-HO patients HOMA-IR remained an independent predictor of increased numbers of circulating CD31+/ Annexin V+ and CD144+/ Annexin V+ EMPs.
Conclusion: in the investigation we found that the increased number of CD31+/Annexin V+ and CD144+/ Annexin V+ EMPs added to the based predictive model (HOMA-IR) may predict transformation of Met-HO into Met-UHO.
References
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Antwi, F., Fazylova, N., Garcon, M.-C., Lopez, L., Rubiano, R., and Slyer, J.T. (2012). The effectiveness of web‐based programs on the reduction of childhood obesity in school‐aged children: A systematic review. JBI Database of Systematic Reviews and Implementation Reports 10, 1-14.
Ashwell, M., Gunn, P., and Gibson, S. (2012). Waist‐to‐height ratio is a better screening tool than waist circumference and BMI for adult cardiometabolic risk factors: systematic review and meta‐analysis. Obesity reviews 13, 275-286.
Basterra-Gortari, F., Bes-Rastrollo, M., Ruiz-Canela, M., Gea, A., and Martinez-Gonzalez, M. (2017). Trends in the prevalence of obesity and diabetes in Spanish adults 1987-2012. Medicina clinica.
Berezin, A., Zulli, A., Kerrigan, S., Petrovic, D., and Kruzliak, P. (2015a). Predictive role of circulating endothelial-derived microparticles in cardiovascular diseases. Clinical biochemistry 48, 562-568.
Berezin, A.E. (2016a). Biomarkers for cardiovascular risk in patients with diabetes (BMJ Publishing Group Ltd and British Cardiovascular Society).
Berezin, A.E. (2016b). Cardiac biomarkers in diabetes mellitus: New dawn for risk stratification? Diabetes & Metabolic Syndrome: Clinical Research & Reviews.
Berezin, A.E., Kremzer, A.A., Berezina, T.A., and Martovitskaya, Y.V. (2015b). Pattern of circulating microparticles in chronic heart failure patients with metabolic syndrome: Relevance to neurohumoral and inflammatory activation. BBA clinical 4, 69-75.
Berezin, A.E., Kremzer, A.A., Berezina, T.A., and Martovitskaya, Y.V. (2016a). The pattern of circulating microparticles in patients with diabetes mellitus with asymptomatic atherosclerosis. Acta clinica Belgica 71, 38-45.
Berezin, A.E., Kremzer, A.A., Cammarota, G., Zulli, A., Petrovic, D., Martell-Claros, N., Sabo, J., and Kruzliak, P. (2016b). Circulating endothelial-derived apoptotic microparticles and insulin resistance in non-diabetic patients with chronic heart failure. Clinical Chemistry and Laboratory Medicine (CCLM) 54, 1259-1267.
Berezin, A.E., Kremzer, A.A., Martovitskaya, Y.V., Berezina, T.A., and Gromenko, E.A. (2016c). Pattern of endothelial progenitor cells and apoptotic endothelial cell-derived microparticles in chronic heart failure patients with preserved and reduced left ventricular ejection fraction. EBioMedicine 4, 86-94.
Berezin, A.E., Kremzer, A.A., Samura, T.A., Berezina, T.A., and Kruzliak, P. (2015c). Immune phenotypes of endothelial-derived microparticles in dysmetabolic patients. Journal of Proteomics & Bioinformatics 8, 60.
Brede, S., Serfling, G., Klement, J., Schmid, S.M., and Lehnert, H. (2016). Clinical Scenario of the Metabolic Syndrome. Visceral medicine 32, 336-341.
Consultation, W.E. (2008). Waist circumference and waist-hip ratio. Report of a WHO Expert Consultation Geneva: World Health Organization, 8-11.
Cuthbertson, D.J., Steele, T., Wilding, J.P., Halford, J.C., Harrold, J.A., Hamer, M., and Karpe, F. (2017). What have human experimental overfeeding studies taught us about adipose tissue expansion and susceptibility to obesity and metabolic complications.
Cvjetkovic, A., Lötvall, J., and Lässer, C. (2014). The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles. Journal of extracellular vesicles 3.
Finucane, M.M., Stevens, G.A., Cowan, M.J., Danaei, G., Lin, J.K., Paciorek, C.J., Singh, G.M., Gutierrez, H.R., Lu, Y., and Bahalim, A.N. (2011). National, regional, and global trends in body-mass index since 1980: systematic analysis of health examination surveys and epidemiological studies with 960 country-years and 9· 1 million participants. The Lancet 377, 557-567.
Flegal, K.M., Carroll, M.D., Ogden, C.L., and Curtin, L.R. (2010). Prevalence and trends in obesity among US adults, 1999-2008. Jama 303, 235-241.
Grundy, S.M., Cleeman, J.I., Daniels, S.R., Donato, K.A., Eckel, R.H., Franklin, B.A., Gordon, D.J., Krauss, R.M., Savage, P.J., and Smith, S.C. (2005). Diagnosis and management of the metabolic syndrome. Circulation 112, 2735-2752.
Grundy, S.M., Cleeman, J.I., Merz, C.N.B., Brewer, H.B., Clark, L.T., Hunninghake, D.B., Pasternak, R.C., Smith, S.C., and Stone, N.J. (2004). A summary of implications of recent clinical trials for the National Cholesterol Education Program Adult Treatment Panel III guidelines (Am Heart Assoc).
Jansen, F., Yang, X., Baumann, K., Przybilla, D., Schmitz, T., Flender, A., Paul, K., Alhusseiny, A., Nickenig, G., and Werner, N. (2015). Endothelial microparticles reduce ICAM‐1 expression in a microRNA‐222‐dependent mechanism. Journal of cellular and molecular medicine 19, 2202-2214.
Kim, H.-N., Kim, S.-H., Eun, Y.-M., and Song, S.-W. (2015). Obesity with metabolic abnormality is associated with the presence of carotid atherosclerosis in Korean men: a cross-sectional study. Diabetology & metabolic syndrome 7, 68.
Lacroix, R., Judicone, C., Mooberry, M., Boucekine, M., Key, N.S., and Dignat‐George, F. (2013). Standardization of pre‐analytical variables in plasma microparticle determination: results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop. Journal of Thrombosis and Haemostasis 11, 1190-1193.
Lee, S.K., Yang, S.-H., Kwon, I., Lee, O., and Heo, J.H. (2014). Role of tumour necrosis factor receptor-1 and nuclear factor-B in production of TNF--induced pro-inflammatory microparticles in endothelial cells. Thrombosis and haemostasis 112, 580-588.
Levey, A.S., Stevens, L.A., Schmid, C.H., Zhang, Y.L., Castro, A.F., 3rd, Feldman, H.I., Kusek, J.W., Eggers, P., Van Lente, F., Greene, T., et al. (2009). A new equation to estimate glomerular filtration rate. Annals of internal medicine 150, 604-612.
Lichtenauer, M., Goebel, B., Fritzenwanger, M., Förster, M., Betge, S., Lauten, A., Figulla, H.-R., and Jung, C. (2015). Simulated temporary hypoxia triggers the release of CD31+/Annexin+ endothelial microparticles: A prospective pilot study in humans. Clinical hemorheology and microcirculation 61, 83-90.
Lindson-Hawley, N., Begh, R., McDermott, M.S., McEwen, A., and Lycett, D. (2013). The importance of practitioner smoking status: A survey of NHS Stop Smoking Service practitioners. Patient education and counseling 93, 139-145.
Martinez, M.C., and Andriantsitohaina, R. (2011). Microparticles in angiogenesis. Circulation Research 109, 110-119.
Matthews, D., Hosker, J., Rudenski, A., Naylor, B., Treacher, D., and Turner, R. (1985). Homeostasis model assessment: insulin resistance and β-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 28, 412-419.
Mause, S.F., and Weber, C. (2010). Microparticles. Circulation research 107, 1047-1057.
Mongraw-Chaffin, M., Foster, M.C., Kalyani, R.R., Vaidya, D., Burke, G.L., Woodward, M., and Anderson, C.A. (2016). Obesity severity and duration are associated with incident metabolic syndrome: Evidence against metabolically healthy obesity from the Multi-Ethnic Study of Atherosclerosis. The Journal of Clinical Endocrinology & Metabolism 101, 4117-4124.
Montoro-García, S., Shantsila, E., Marín, F., Blann, A., and Lip, G.Y. (2011). Circulating microparticles: new insights into the biochemical basis of microparticle release and activity. Basic research in cardiology 106, 911-923.
Orozco, A.F., and Lewis, D.E. (2010). Flow cytometric analysis of circulating microparticles in plasma. Cytometry Part A 77, 502-514.
Rautou, P.-E., Vion, A.-C., Amabile, N., Chironi, G., Simon, A., Tedgui, A., and Boulanger, C.M. (2011). Microparticles, vascular function, and atherothrombosis. Circulation research 109, 593-606.
Rey‐López, J., Rezende, L., Pastor‐Valero, M., and Tess, B. (2014). The prevalence of metabolically healthy obesity: a systematic review and critical evaluation of the definitions used. Obesity reviews 15, 781-790.
Ryden, L., Grant, P.J., Anker, S.D., Berne, C., Cosentino, F., Danchin, N., Deaton, C., Escaned, J., Hammes, H.P., Huikuri, H., et al. (2013). ESC Guidelines on diabetes, pre-diabetes, and cardiovascular diseases developed in collaboration with the EASD: the Task Force on diabetes, pre-diabetes, and cardiovascular diseases of the European Society of Cardiology (ESC) and developed in collaboration with the European Association for the Study of Diabetes (EASD). European heart journal 34, 3035-3087.
Sterling, S.A., Jones, A.E., and Cox, R.D. (2016). Longitudinal Trends in the Prevalence of Diabetes Mellitus in an Urban Emergency Department. Southern medical journal 109, 222-227.
Sturm, R. (2007). Increases in morbid obesity in the USA: 2000–2005. Public health 121, 492-496.
Tetta, C., Bruno, S., Fonsato, V., Deregibus, M.C., and Camussi, G. (2011). The role of microvesicles in tissue repair. Organogenesis 7, 105-115.
Valdés, S., García-Torres, F., Maldonado-Araque, C., Goday, A., Calle-Pascual, A., Soriguer, F., Castaño, L., Catalá, M., Gomis, R., and Rojo-Martínez, G. (2014). Prevalence of obesity, diabetes and other cardiovascular risk factors in Andalusia (southern Spain). Comparison with national prevalence data. The Di@ bet. es study. Revista Española de Cardiología (English Edition) 67, 442-448.
Vanuzzo, D., Pilotto, L., Mirolo, R., and Pirelli, S. (2008). Cardiovascular risk and cardiometabolic risk: an epidemiological evaluation. Giornale italiano di cardiologia (2006) 9, 6S-17S.
Williams, L. (2002). Third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (Adult Treatment Panel III) final report. Circulation 106, 3143-3143.
Alexandru, N., Badila, E., Weiss, E., Cochior, D., Stępień, E., and Georgescu, A. (2016). Vascular complications in diabetes: Microparticles and microparticle associated microRNAs as active players. Biochemical and biophysical research communications 472, 1-10.
Antwi, F., Fazylova, N., Garcon, M.-C., Lopez, L., Rubiano, R., and Slyer, J.T. (2012). The effectiveness of web‐based programs on the reduction of childhood obesity in school‐aged children: A systematic review. JBI Database of Systematic Reviews and Implementation Reports 10, 1-14.
Ashwell, M., Gunn, P., and Gibson, S. (2012). Waist‐to‐height ratio is a better screening tool than waist circumference and BMI for adult cardiometabolic risk factors: systematic review and meta‐analysis. Obesity reviews 13, 275-286.
Basterra-Gortari, F., Bes-Rastrollo, M., Ruiz-Canela, M., Gea, A., and Martinez-Gonzalez, M. (2017). Trends in the prevalence of obesity and diabetes in Spanish adults 1987-2012. Medicina clinica.
Berezin, A., Zulli, A., Kerrigan, S., Petrovic, D., and Kruzliak, P. (2015a). Predictive role of circulating endothelial-derived microparticles in cardiovascular diseases. Clinical biochemistry 48, 562-568.
Berezin, A.E. (2016a). Biomarkers for cardiovascular risk in patients with diabetes (BMJ Publishing Group Ltd and British Cardiovascular Society).
Berezin, A.E. (2016b). Cardiac biomarkers in diabetes mellitus: New dawn for risk stratification? Diabetes & Metabolic Syndrome: Clinical Research & Reviews.
Berezin, A.E., Kremzer, A.A., Berezina, T.A., and Martovitskaya, Y.V. (2015b). Pattern of circulating microparticles in chronic heart failure patients with metabolic syndrome: Relevance to neurohumoral and inflammatory activation. BBA clinical 4, 69-75.
Berezin, A.E., Kremzer, A.A., Berezina, T.A., and Martovitskaya, Y.V. (2016a). The pattern of circulating microparticles in patients with diabetes mellitus with asymptomatic atherosclerosis. Acta clinica Belgica 71, 38-45.
Berezin, A.E., Kremzer, A.A., Cammarota, G., Zulli, A., Petrovic, D., Martell-Claros, N., Sabo, J., and Kruzliak, P. (2016b). Circulating endothelial-derived apoptotic microparticles and insulin resistance in non-diabetic patients with chronic heart failure. Clinical Chemistry and Laboratory Medicine (CCLM) 54, 1259-1267.
Berezin, A.E., Kremzer, A.A., Martovitskaya, Y.V., Berezina, T.A., and Gromenko, E.A. (2016c). Pattern of endothelial progenitor cells and apoptotic endothelial cell-derived microparticles in chronic heart failure patients with preserved and reduced left ventricular ejection fraction. EBioMedicine 4, 86-94.
Berezin, A.E., Kremzer, A.A., Samura, T.A., Berezina, T.A., and Kruzliak, P. (2015c). Immune phenotypes of endothelial-derived microparticles in dysmetabolic patients. Journal of Proteomics & Bioinformatics 8, 60.
Brede, S., Serfling, G., Klement, J., Schmid, S.M., and Lehnert, H. (2016). Clinical Scenario of the Metabolic Syndrome. Visceral medicine 32, 336-341.
Consultation, W.E. (2008). Waist circumference and waist-hip ratio. Report of a WHO Expert Consultation Geneva: World Health Organization, 8-11.
Cuthbertson, D.J., Steele, T., Wilding, J.P., Halford, J.C., Harrold, J.A., Hamer, M., and Karpe, F. (2017). What have human experimental overfeeding studies taught us about adipose tissue expansion and susceptibility to obesity and metabolic complications.
Cvjetkovic, A., Lötvall, J., and Lässer, C. (2014). The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles. Journal of extracellular vesicles 3.
Finucane, M.M., Stevens, G.A., Cowan, M.J., Danaei, G., Lin, J.K., Paciorek, C.J., Singh, G.M., Gutierrez, H.R., Lu, Y., and Bahalim, A.N. (2011). National, regional, and global trends in body-mass index since 1980: systematic analysis of health examination surveys and epidemiological studies with 960 country-years and 9· 1 million participants. The Lancet 377, 557-567.
Flegal, K.M., Carroll, M.D., Ogden, C.L., and Curtin, L.R. (2010). Prevalence and trends in obesity among US adults, 1999-2008. Jama 303, 235-241.
Grundy, S.M., Cleeman, J.I., Daniels, S.R., Donato, K.A., Eckel, R.H., Franklin, B.A., Gordon, D.J., Krauss, R.M., Savage, P.J., and Smith, S.C. (2005). Diagnosis and management of the metabolic syndrome. Circulation 112, 2735-2752.
Grundy, S.M., Cleeman, J.I., Merz, C.N.B., Brewer, H.B., Clark, L.T., Hunninghake, D.B., Pasternak, R.C., Smith, S.C., and Stone, N.J. (2004). A summary of implications of recent clinical trials for the National Cholesterol Education Program Adult Treatment Panel III guidelines (Am Heart Assoc).
Jansen, F., Yang, X., Baumann, K., Przybilla, D., Schmitz, T., Flender, A., Paul, K., Alhusseiny, A., Nickenig, G., and Werner, N. (2015). Endothelial microparticles reduce ICAM‐1 expression in a microRNA‐222‐dependent mechanism. Journal of cellular and molecular medicine 19, 2202-2214.
Kim, H.-N., Kim, S.-H., Eun, Y.-M., and Song, S.-W. (2015). Obesity with metabolic abnormality is associated with the presence of carotid atherosclerosis in Korean men: a cross-sectional study. Diabetology & metabolic syndrome 7, 68.
Lacroix, R., Judicone, C., Mooberry, M., Boucekine, M., Key, N.S., and Dignat‐George, F. (2013). Standardization of pre‐analytical variables in plasma microparticle determination: results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop. Journal of Thrombosis and Haemostasis 11, 1190-1193.
Lee, S.K., Yang, S.-H., Kwon, I., Lee, O., and Heo, J.H. (2014). Role of tumour necrosis factor receptor-1 and nuclear factor-B in production of TNF--induced pro-inflammatory microparticles in endothelial cells. Thrombosis and haemostasis 112, 580-588.
Levey, A.S., Stevens, L.A., Schmid, C.H., Zhang, Y.L., Castro, A.F., 3rd, Feldman, H.I., Kusek, J.W., Eggers, P., Van Lente, F., Greene, T., et al. (2009). A new equation to estimate glomerular filtration rate. Annals of internal medicine 150, 604-612.
Lichtenauer, M., Goebel, B., Fritzenwanger, M., Förster, M., Betge, S., Lauten, A., Figulla, H.-R., and Jung, C. (2015). Simulated temporary hypoxia triggers the release of CD31+/Annexin+ endothelial microparticles: A prospective pilot study in humans. Clinical hemorheology and microcirculation 61, 83-90.
Lindson-Hawley, N., Begh, R., McDermott, M.S., McEwen, A., and Lycett, D. (2013). The importance of practitioner smoking status: A survey of NHS Stop Smoking Service practitioners. Patient education and counseling 93, 139-145.
Martinez, M.C., and Andriantsitohaina, R. (2011). Microparticles in angiogenesis. Circulation Research 109, 110-119.
Matthews, D., Hosker, J., Rudenski, A., Naylor, B., Treacher, D., and Turner, R. (1985). Homeostasis model assessment: insulin resistance and β-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 28, 412-419.
Mause, S.F., and Weber, C. (2010). Microparticles. Circulation research 107, 1047-1057.
Mongraw-Chaffin, M., Foster, M.C., Kalyani, R.R., Vaidya, D., Burke, G.L., Woodward, M., and Anderson, C.A. (2016). Obesity severity and duration are associated with incident metabolic syndrome: Evidence against metabolically healthy obesity from the Multi-Ethnic Study of Atherosclerosis. The Journal of Clinical Endocrinology & Metabolism 101, 4117-4124.
Montoro-García, S., Shantsila, E., Marín, F., Blann, A., and Lip, G.Y. (2011). Circulating microparticles: new insights into the biochemical basis of microparticle release and activity. Basic research in cardiology 106, 911-923.
Orozco, A.F., and Lewis, D.E. (2010). Flow cytometric analysis of circulating microparticles in plasma. Cytometry Part A 77, 502-514.
Rautou, P.-E., Vion, A.-C., Amabile, N., Chironi, G., Simon, A., Tedgui, A., and Boulanger, C.M. (2011). Microparticles, vascular function, and atherothrombosis. Circulation research 109, 593-606.
Rey‐López, J., Rezende, L., Pastor‐Valero, M., and Tess, B. (2014). The prevalence of metabolically healthy obesity: a systematic review and critical evaluation of the definitions used. Obesity reviews 15, 781-790.
Ryden, L., Grant, P.J., Anker, S.D., Berne, C., Cosentino, F., Danchin, N., Deaton, C., Escaned, J., Hammes, H.P., Huikuri, H., et al. (2013). ESC Guidelines on diabetes, pre-diabetes, and cardiovascular diseases developed in collaboration with the EASD: the Task Force on diabetes, pre-diabetes, and cardiovascular diseases of the European Society of Cardiology (ESC) and developed in collaboration with the European Association for the Study of Diabetes (EASD). European heart journal 34, 3035-3087.
Sterling, S.A., Jones, A.E., and Cox, R.D. (2016). Longitudinal Trends in the Prevalence of Diabetes Mellitus in an Urban Emergency Department. Southern medical journal 109, 222-227.
Sturm, R. (2007). Increases in morbid obesity in the USA: 2000–2005. Public health 121, 492-496.
Tetta, C., Bruno, S., Fonsato, V., Deregibus, M.C., and Camussi, G. (2011). The role of microvesicles in tissue repair. Organogenesis 7, 105-115.
Valdés, S., García-Torres, F., Maldonado-Araque, C., Goday, A., Calle-Pascual, A., Soriguer, F., Castaño, L., Catalá, M., Gomis, R., and Rojo-Martínez, G. (2014). Prevalence of obesity, diabetes and other cardiovascular risk factors in Andalusia (southern Spain). Comparison with national prevalence data. The Di@ bet. es study. Revista Española de Cardiología (English Edition) 67, 442-448.
Vanuzzo, D., Pilotto, L., Mirolo, R., and Pirelli, S. (2008). Cardiovascular risk and cardiometabolic risk: an epidemiological evaluation. Giornale italiano di cardiologia (2006) 9, 6S-17S.
Williams, L. (2002). Third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (Adult Treatment Panel III) final report. Circulation 106, 3143-3143.
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2017-01-27
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Circulating apoptotic endothelial cell-derived microparticles are predicted metabolically unhealthy obesity. (2017). Biomedical Research and Therapy, 4(1), 1110-1128. https://doi.org/10.15419/bmrat.v4i1.147
