Preliminary evaluation of intravenous infusion and intra-pancreatic injection of human umbilical cord blood-derived mesenchymal stem cells for the treatment of diabetic mice

Authors

  • Ngoc Kim Phan Laboratory of Stem Cell Research and Application, University of Sciences, Vietnam National University, Ho Chi Minh City, Viet Nam
  • Thuy Thanh Duong Laboratory of Stem Cell Research and Application, University of Sciences, Vietnam National University, Ho Chi Minh City, Viet Nam
  • Truc Le-Buu Pham Laboratory of Stem Cell Research and Application, University of Sciences, Vietnam National University, Ho Chi Minh City, Viet Nam
  • Loan Thi-Tung Dang Laboratory of Stem Cell Research and Application, University of Sciences, Vietnam National University, Ho Chi Minh City, Viet Nam
  • Anh Nguyen-Tu Bui Laboratory of Stem Cell Research and Application, University of Sciences, Vietnam National University, Ho Chi Minh City, Viet Nam
  • Vuong Minh Pham Laboratory of Stem Cell Research and Application, University of Sciences, Vietnam National University, Ho Chi Minh City, Viet Nam
  • Nhat Chau Truong Laboratory of Stem Cell Research and Application, University of Sciences, Vietnam National University, Ho Chi Minh City, Viet Nam
  • Phuc Van Pham Laboratory of Stem Cell Research and Application, University of Sciences, Vietnam National University, Ho Chi Minh City, Viet Nam

Keywords:

Type 1 diabetes mellitus, umbilical cord blood, mesenchymal stem cells, intravenous transplantation, intra-pancreatic injection

Abstract

Type 1 diabetes mellitus is characterized by the destruction of pancreatic islet beta cells, which leads to insulin insufficiency, hyperglycemia, and reduced metabolic glucose level. Insulin replacement is the current standard therapy for type 1 diabetes mellitus but has several limitations. Pancreatic islet transplantation can result in the production of exogenous insulin, but its use is limited by immune-rejection and donor availability. Recent studies have shown that mesenchymal stem cells (MSCs) can trans-differentiate into insulin-producing cells (IPCs), which could be utilized for diabetes mellitus treatment. Previously published reports have demonstrated that MSC or IPC transplantation could produce significant improvement in mouse models of diabetes mellitus. This study was aimed at determining the effects of two different methods of MSC transplantation on the efficacy of diabetes mellitus treatment in mouse models. The MSCs were isolated from umbilical cord blood and were proliferated following a previously published procedure. Diabetes mellitus was induced in mice by streptozotocin (STZ) injection. Thirty days after transplantation, the weight of the mice treated by intra-venous infusion and intra-pancreatic injection was found to be 22% and 14% higher than that of the un-treated mice. The blood glucose concentrations in both intra-venous infusion and intra-pancreatic injection groups decreased and remained more stable than those in the control group. Moreover, insulin was detected in the serum of the treated mice, and the pancreas also showed gradual recovery. Based on the results of this preliminary investigation, intra-venous infusion seems more suitable than intra-pancreatic injection for MSC transplantation for diabetes mellitus treatment.

 

Published

2014-08-08

Issue

Section

Original Research

How to Cite

Preliminary evaluation of intravenous infusion and intra-pancreatic injection of human umbilical cord blood-derived mesenchymal stem cells for the treatment of diabetic mice. (2014). Biomedical Research and Therapy, 1(03), 98-105. https://preservation.bmrat.org/index.php/BMRAT/article/view/27

Similar Articles

1-10 of 625

You may also start an advanced similarity search for this article.