ID: 1060 Organic-inorganic Biomaterials promote mesenchymal stem cell proliferation and differentiation for long-term cultivation
DOI:
https://doi.org/10.15419/bmrat.v4iS.334Keywords:
Biology, bioactive glass, denuded amnion, graphene oxide, hydroxyapatite, long-term screening, Wharton's jelly derived mesenchymal stem cellAbstract
Background: A nifty propagating of mesenchymal stem cell (MSCs) diligence has germinated all over the world by innovative investigators. However, the clinical and basic research applications of MSC requires novel finding biomaterials interfacial interaction especially in sustainable the morphology, physiology, multipotent and phenotypically in long-term cultivation. A prominent of biomaterials benefit to MSCs culture has triggered the multitudinous field especially in regenerative medicine. In order to hinder the deprivation of MSCs in purity and potency, the alternative cell-substrate materials of MSCs culture is essentially to be discovered.
This has instigated the idea to encountered the method of screening libraries organic and inorganics biomaterials in bio-adhesively, free ethically, and sustainability to support the morphologically, physiologically, multi-potent and phenotypically of substrates coating cover slip.
Methods: Libraries of inorganic biomaterials substrates have been collected from co-researcher to conduct the initial screening phase of 100 myriad fabrications of substrates whereas enumerated as a Graphene Oxide (GO), Hydroxyapatite (HAp), and Bioactive Glass (BAG) coated cover slip and discs also several organic biomaterials. Wharton’s Jelly derived Mesenchymal Stem Cells (WJMSCs) and Denuded Amnion Mesenchymal Stem Cells (AMMSCs) have been seeded on each substrate in the 48-well plate. Top four leading substrates have been selected for further cultivation until up to 5 passage (>P5) for long term screening known as scaling up phase. Several parameters such as cell attachment, cell viability, kinetic growth, cell-materials osteogenic and adipogenic differentiation and cell phenotype have been analyzed. Top one cell-material culture will go forward to further long-term cultivation up to Passage 10(>P10).
Results: Morphologically and phenotypically demonstrated that GOy1WJMSC showed the significance result among others.
References
2-Ghaemi, S.R., Harding, F.J., Delalat, B., Gronthos, S., Voelcker, N.H. 2013. Exploring the mesenchymal stem cell niche using high throughput screening. Biomaterials 34; 7601-7615.
3.Lijima, K., Suzuki, R., Lizuka, A., Ueno-Yokohata, H., Kiyokawa, N., Hashizume, M. 2016. Surface functionalization of tissue culture polystyrene plates with hydroxyapatite under body fluid conditions and its effect on differentiation behaviors of mesenchymal stem cells. Colloids and Surfaces B: Biointerfaces 147; 353-359.
4-Ribeiro, A., Laranjeira, P., Mendes, S>, Velada, I., Leite, C., Andrade, P., Santos, F., Henriques, A., Graos, M., Cardoso, C.M.P., Martinho, A., Pais, M.L., Lobato da silva, C., Cabral, J., Trindase, H., Paiva, A. 2013. Mesenchymal stem cells from umbilical cord matrix, adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B, natural killer and T cells. Stem Cell Research & Therapy, 4; 125.
5.Zeddou, M., Briquet, A., Relic, B., Josse, C., Malaise, M.G., Gothot, A., Lechanteur, C., Beguin, Yves. 2010. The umbilical cord matrix is a better source of mesenchymal stem cells (MSC) than the umbilical cord blood. Cell Biology International, 34; 693-701.
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Copyright The Author(s) 2017. This article is published with open access by BioMedPress. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
