Ex-vivo cytotoxic, antibacterial and DPPH free radical scavenging assay with ethanolic leaf extract of Glycosmis pentaphylla to justify its traditional use

Abstract

Aim: Glycosmis pentaphylla belongs to the family Rutaceae. It is a shrub and locally common in the treatment of hepatic impairment. We have designed this study to provide a scientific basis with the traditional use of leaf of G. pentaphylla in the treatment of hepatitis.

Methods: The well-established DPPH free radical scavenging activity was tested for antioxidant property evaluation. On the other hand, disk diffusion and brine shrimp method was respectively used to determine antibacterial and cytotoxic activity.

Results & Discussion: In the evaluation of antioxidant property IC₅₀ found 204.91 ± 2.223 µg/ml, in cytotoxicity testing, it is found that the plant part shows 30.49 ± 1.976 µg/ml of LC₅₀. The ethanolic extract of G. pentaphylla leaves also have efficiency in bacterial growth inhibition; this extract is effective against for both gram, negative and positive. The zone of inhibition at 500 µg/ml dose in E. coli and C. albican culture was 18 mm and 15 mm, respectively. In thin layer chromatography analysis, we found presence of couple of non-polar and polar component, presence of three non-chromatophoric component are also evident.

Conclusion: Appropriate isolation and identification of mechanism is suggested in further study.