Collagenolytic activitiy in tissue extract of Parborlasia corrugatus from antarctic region

Authors

  • Nataliia Raksha ESC ‘Institute of Biology’ Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
  • Dmitrii Gladun Taras Shevchenko National University of Kyiv, ESC ‘Institute of Biology’, 64/13, Volodymyrska Str., Kyiv 01601, Ukraine
  • Oleksii Savchuk Taras Shevchenko National University of Kyiv, ESC ‘Institute of Biology’, 64/13, Volodymyrska Str., Kyiv 01601, Ukraine
  • Lydmila Ostapchenko ESC ‘Institute of Biology’ Taras Shevchenko National University of Kyiv, Kyiv, Ukraine

Abstract

Marine organisms have been recognized as rich sources of bioactive compounds with valuable biotechnology potential. Enzymes extracted from marine hydrobionts have gained much attention because of their unique quite specific properties that determined their profound applications in chemical, medical, food industries and molecular biology experiments. In this regard, our work focused on investigation of proteolytic potential of marine hydrobionts. At first, tissue extract of Antarctic hydrobiont Parborlasia corrugatus was separated by gel filtration chromatography on a Superdex-75 PG. Further zymography with using gelatin as substrate revealed the presence of clear band that can indicate about active enzymes. It had been shown the presence of collagenolytic activity in all eight fractions obtained after chromatographic separation of tissue extract. Trypsin-like (L-BApNA hydrolyzing) was found only in first fraction. Our results let us assume that P. corrugatus can be regarded as potential source of enzymes for practical use.

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Published

2015-09-10

Issue

Vol. 2 No. 9 (2015)

Section

Original Research

License

Copyright The Author(s) 2017. This article is published with open access by BioMedPress. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

How to Cite

Collagenolytic activitiy in tissue extract of Parborlasia corrugatus from antarctic region. (2015). Biomedical Research and Therapy, 2(9), 354-358. https://preservation.bmrat.org/index.php/BMRAT/article/view/59