A safety evaluation of adipose derived stem cells

Authors

  • Ngoc Bich Vu Laboratory of Stem cell Research and Application, University of Science, VNU-HCMC, Viet Nam
  • Van Ngoc Le Trinh Laboratory of Stem cell Research and Application, University of Science, VNU-HCMC, Viet Nam
  • Lan Thi Phi Laboratory of Stem cell Research and Application, University of Science, VNU-HCMC, Viet Nam
  • Trieu Le Hoang Vo Laboratory of Stem cell Research and Application, University of Science, VNU-HCMC, Viet Nam
  • Thuy Thi Thanh Dao Laboratory of Stem cell Research and Application, University of Science, VNU-HCMC, Viet Nam
  • Ngoc Kim Phan Laboratory of Stem cell Research and Application, University of Science, VNU-HCMC, Viet Nam
  • Van Thanh Ta Ha Noi Medical University, Ha Noi, Viet Nam
  • Phuc Van Pham Laboratory of Stem cell Research and Application, University of Science, VNU-HCMC, Viet Nam

Abstract

Adipose tissue contains a large numbers of stem cells. Adipose-derived stem cells (ADSC) employed in regenerative medicine. This study aimed to isolate ADSC and evaluate the safety of ADSC in mice model. Stromal vascular fraction (SVF) was collected from adipose tissue by collagenase. Then, ADSCs were isolated from SVFs by in vitro culture. ADSCs were evaluated their stemness in vitro by self-renewal potential, differentiation potential into osteoblasts, adipocytes, and chondroblasts as well as expression of some specific markers. Finally, the ADSCs were evaluated tumor formation in vivo in athymic mouse. Results showed that 100% samples of ADSCs were developed well and maintained homogeneous up to passage 10th. ADSCs were completely sterilized and could not cause tumors in athymic mouse. These initial results showed that ADSCs were safety for stem cell therapy.

Downloads

Published

2015-09-13

Issue

Vol. 2 No. 9 (2015)

Section

Original Research

License

Copyright The Author(s) 2017. This article is published with open access by BioMedPress. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

How to Cite

A safety evaluation of adipose derived stem cells. (2015). Biomedical Research and Therapy, 2(9), 359-365. https://preservation.bmrat.org/index.php/BMRAT/article/view/61