Investigation of In-vivo Anti-diarrheal and In-vitro Antihelminthic properties of Methanolic Leaves Extract of Dalbergia stipulacea Roxb

Abstract

Aim: This study is designed to identify and evaluate the pharmacological property of leaf extract of D. stipulacea, commonly known as Horoiludi in Bangladesh.

Materials and Methods: The anti-diarrheal activity was estimated using Galvez et al. method with a simple modification. The testing extract was compared to standard Loperamide (5 mg/kg, p.o.), while the extract had two different concentration 200 mg/kg and 400 mg/kg. On the other hand antihelminthic property of this plant was investigated using Panagrellus redivivus worms.

Result: At maximum concentration (400 mg/kg) it inhibited 42.22 % and loperamide was found to inhibit 62.23 %. The one-way ANOVA for this testing result in P < 0.0001, which means that the investigation result have significant importance in consideration of statistical analysis. The antihelminthic testing also results in a dose dependent manner. At highest dose (2.5 mg) the extract paralyzed experimental worm in 5.5 ± 1.323 min and it died in 23.93 ± 1.901 min.

Conclusion: Based on the data analysis obtained from the study, it can be concluded that the plant part has good anti-diarrheal activity as well as antihelminthic property. We need to identify its actual molecule through further processing.