Green Synthesis of RES-CMCS: A Promising Modulator of the GLUT-4/Leptin Signaling Pathway in HFD-induced Insulin Resistance
DOI:
https://doi.org/10.15419/bmrat.v9i7.753Keywords:
Resveratrol, RES-CMCS, insulin, total cholesterol, leptin, GLUT-4, rats and histopathologyAbstract
Introduction: Resveratrol (RES) is a multi-biofunctional compound found in grapes and mulberries. The present investigation was aimed at the green synthesis of resveratrol-carboxymethyl nanoparticles using low viscosity chitosan (RES-CMCS) and evaluation of their antidiabetic and antiobesity activity.
Methods: The obtained RES-CMCS was analyzed via measurement of zeta potential, particle size, morphology, and entrapment effectiveness. Its antidiabetic and antiobesity activities were also examined in an obese rat model.
Results: The mean size of the RES-CMCS nanoparticles was 54.7 nm, the zeta potential was (-) 59.4 mV, and the entrapment effectiveness was 85.46%, with spherical nanoparticle morphology detected. In addition, treatment of obese diabetic rats with RES-CMCS (25 and 50 mg) as well as metformin (500 mg/kg.b.w) resulted in the normalization of several physiological parameters including body weight, levels of blood glucose, lipid profile, oxidative stress indicators, and expression of GLUT-4 and leptin genes.
Conclusions: The findings show the potential for RES-CMCS as a new pharmaceutical medication method for the treatment of obesity via modulation of antioxidant enzymes and expression of GLUT-4 and leptin.
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Copyright The Author(s) 2017. This article is published with open access by BioMedPress. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
