Characterization of white matter branching in human cerebella: quantitative morphological assessment and fractal analysis of skeletonized MR images

Abstract

Introduction: The aim of the present study was to investigate branching characteristics of the human cerebellar white matter by the means of findings obtained from the quantitative morphological assessment and fractal analysis of the skeletonized MR images of the human cerebellum.

Methods: Thirty individuals with no apparent brain pathology (15 males and 15 females, ranging from 18 - 30 years of age) participated in this study. Their normal T2-weighted MR images of the cerebellar vermis (midsagittal plane) were examined. The skeletonizing procedure and subsequent quantitative morphological assessment of the acquired skeletonized MR images were performed. The following parameters were determined: the number of branches, the number of junctions, the amount of end-point voxels, junction voxels and slab voxels, the average and maximum branch lengths, the longest-shortest patch length, and the number of triple and quadruple points. Additionally, the individual branches of the obtained digital skeletons of the cerebellar white matter were examined and the following parameters were assessed: branch length variability, Euclidean distance, and branch length/Euclidean distance ratio. A fractal analysis was performed using the box counting method prior to and after the MR image skeletonizing procedure. The values of the fractal dimensions (FD) of both skeletonized and non-skeletonized MR images were calculated.

Results: It was established that the cerebella, which had the maximum values of the FD, possessed a large number of small branches approximately equal in length and which were connected by numerous junctions, forming numerous endpoints. Those cerebella, which had higher values of the average branch length and greater branch length variability, showed lower values of the FD. The key characteristics of the digital skeleton that determined the values of the FD of the cerebellum and its skeletonized MR images were the number of branches and the number of junctions that had the strongest correlational relationships with the FD of the skeletonized MR images. We submitted a proposition to consider the number of branches and amount of junctions as a diagnostic criterion in the determination of normal values of the FD.

Conclusions: The obtained data can be used as diagnostic criteria in assessment of the morphofunctional state of the cerebellum using magnetic resonance imaging (MRI) technique.